Check this option to use a custom threshold for drawing reference lines for Bilirubin (on the X axis) and for Alanine Aminotransferase (on the Y axis) of the plot.When this box is checked, you can change the values that you want to draw reference lines using the Set reference line for bilirubin and Set reference line for transaminase tests options.If left unchecked, traditional Hy’s Law reference lines (twice the upper limit of normal (ULN) for bilirubin and three times the ULN for alanine aminotransferase) are drawn.Note : When this box is checked, you must specify a set reference line for both bilirubin and alanine aminotransferase.