Use this field to specify the design variables that distinguish sets of columns (samples) considered to be distinct groups for analysis.
If only one analysis group is to be used as the control samples (one set of control sample reference computation method), then the values for each column in this parameter field that indicate controls should be specified in the Control Levels field.
If multiple sets of controls are to be used for different groups of samples, then a subset of the columns specified here need to be specified to define the analysis groups that are control sets in the Variables Defining Control Sets field.
For paired sample subtraction, this field should contain two variables, one identifying the paired samples and the second that identifies control measurements within the paired samples. The control identifying variable should the only variable specified in the Variables Defining Control Sets field.
Note: For more complex designs, such as nested designs or where the value of the control changes across Analysis groups, it might be necessary to construct a new column. This column is to be included in this field and in the Variables Defining Control Sets field, in the EDDS, to indicate the analysis groups that should be used as respective controls for the groups’ experimental samples.
All of the variables in the data set are displayed in the Available Variables field.
|
Highlight one or more variables. To highlight multiple variables at once, hold down while left-clicking on them.
|
|
|
Click
 to add the highlighted variable(s) to the Variables Defining Analysis Groups field, as shown below: |
