The Differences Data Set can be created using the Difference Chooser process. Both an Effect variable and variables to specify the levels of the Effects that you want to calculate differences between are required. A variable named Reverse is also required if you would like to reverse the direction of the differences.Important: The parameters settings (Fixed Effects for Differential Expression, Select Comparison Set for Differential Expression Tests, Control Levels for Differential Expression Comparisons) that were used to create this data set in Difference Chooser must match the LSMean parameter settings in this process.You can launch the Difference Chooser process with the EDDS, fixed effects, and output data set already specified by clicking .