Positive Control Method Select the method to use for computing the positive normalization factor. Positive control normalization, also known as code-count normalization, uses a series of exogenous probes (genes) of known concentration. Observed variation for these genes can be attributed specifically to the assay. Normalization occurs by summarizing (i.e. mean or geometric mean) the positive control counts and adjusting samples by a factor relative to other samples. The methods available in this process are summarized in the following table: Method Description Geometric Mean The factor used for normalization is the geometric mean of the positive controls for each sample. Mean The factor used for normalization is the arithmetic mean of the positive controls for each sample. To Specify the Method to Use for Computing the Normalization Factor: Select Positive Control for the Control Gene Normalization. Click the Positive Control Method drop-down menu. Click the desired method.
