Specify the subset of fixed effects for which to construct least squares means (LSMeans). LSMeans are the same as simple effect
mean when the experimental design is
balanced. When it is
unbalanced, they are estimates of effect means that would have been observed if the design had been balanced. The differences between LSMeans are also computed, and these differences often represent the experimental hypotheses of interest. A separate
Volcano Plot is constructed for each difference, and parallel coordinate plots and
hierarchical clustering are constructed based on raw and standardized values of each LSMeans level that has a statistically significant difference.