Select Method

Use the drop-down option to select a method to calculate a subset of features that exhibit high cell-to-cell variation in the data set.

It has been reported that focusing on that exhibit high cell-to-cell variation in downstream analysis helps to highlight biological signal in single-cell data sets.

Two methods, Dispersion and VST, are included in this workflow. The Dispersion method is based on the mean.var. plot in the Seurat package. VST is based on a Loess fitting method which is also described in Seurat.

Please check https://satijalab.org/seurat/vignettes.html and Comprehensive Integration of Single-Cell Data at https://www.cell.com/cell/fulltext/S0092-8674(19)30559-8 for more information.