The Pleiotropic Association process tests for association between multiple traits (separately and jointly) and SNP genotypes or alleles from a single SNP at a time. Two types of analyses can be performed: an ANOVA based on SNP genotypes or a regression testing for a linear trend of SNP alleles for the single-trait tests, and multivariate versions of these models (MANOVAs)are used for testing the traits simultaneously. Adjustments can be made for quantitative or qualitative covariates. P-values from these tests, with adjustments applied if requested, are plotted along the marker map. Venn diagrams can be viewed to compare significant SNPs between the single-trait and multiple-trait tests.
You should refer to the GLM procedure in the SAS/STAT User's Guide for more information.
One Input Data Set is needed for this process. The samplegmdata.sas7bdat data set used in the following example was computer generated and consists of 1000 rows of individuals with 130 columns corresponding to data on these individuals. There are 2 categorical phenotypic variables (sex and disease status) and 4 quantitative phenotypic variables (Qtrt1, Qtrt2, Qtrt3, and Qtrt4). Genotypes for 60 different markers are presented in the two-column allelic format (ma1 — ma120). This data set is partially shown below.
Note that this is a wide data set; phenotypes and markers are listed in columns, whereas individuals are listed in rows.
The second, optional, data set is the Annotation Data Set. This data set contains information, such as gene identity or chromosomal location, for each of the markers. The annotation data set used in this example, the samplemap data set, was computer generated and identifies markers, location and gene identities. A portion of this data set is illustrated below. This data set is a tall data set; each row corresponds to a different marker.
Note: The top-to-bottom order of the rows in the annotation data set matches the left-to-right order of the columns in the input data set. This correspondence is required for markers to be matched appropriately.
Both data sets are described in Data Sets Used in JMP Genomics Processes and are included in the Sample Data folder.
For detailed information about the files and data sets used or created by JMP Life Sciences software, see Files and Data Sets.
The output generated by this process is summarized in a Tabbed report. Refer to the Pleiotropic Association output documentation for detailed descriptions and guides to interpreting your results.