Variable Gene Selection Method

Use this option to select the method to calculate a subset of features that exhibit high cell-to-cell variation in the dataset. It has been reported that focusing on these genes in downstream analysis helps to highlight biological signal in single-cell datasets.

.Two methods are available:

Option:	Description:
Dispersion	Dispersion method is based on the log(variance/mean) plot in the Seurat R package1 https://satijalab.org/seurat/vignettes.html. It uses a relatively simple method to calculate gene variation while controlling for the strong relationship between variability and average expression.
VST	VST (Variance-Stabilizing Transformation) uses Loess regression as a normalization method to control for the mean-variance relationship. It is also included in the the Seurat R package.

Please see Stuart, T. et al. 2019. Comprehensive Integration of Single-Cell Data. Cell 177:1888-1902. for more information.

To Specify the Gene Selection Method:

8

Select the method using the drop-down menu.