Positive Control Method

Select the method to use for computing the positive normalization factor.

Positive control normalization, also known as code-count normalization, uses a series of exogenous probes (genes) of known concentration. Observed variation for these genes can be attributed specifically to the assay. Normalization occurs by summarizing (i.e. mean or geometric mean) the positive control counts and adjusting samples by a factor relative to other samples.

The methods are summarized in the following table:

Method	Description
Geometric Mean	The factor used for normalization is the geometric mean of the positive controls for each sample.
Mean	The factor used for normalization is the arithmetic mean of the positive controls for each sample.

To Specify the Method to Use for Computing the Normalization Factor:

8

Select the Positive Control radio button for Probe Normalization.

8

Click the Positive Control Method drop-down menu.

8

Click the desired method.