When you are using one set of control samples, you can type the levels to be used as a control in this field. Wrap each level in double quotation marks ( “” ) and separate the levels by spaces ( ).Using the Drosophila Aging example, if the Variables Defining Analysis Groups are Line , Sex , and Age , then specify:When using multiple sets of control samples, type the values of the Variables Defining Control Sets (wrapped in double quotation marks ) that indicate the analysis groups that make up the control samples.Important : This field is required when using multiple control groups.If you leave this field blank , the first sorted level for each group variable is used as the control when using one set of control samples.