Use this field to specify the design
variables
that distinguish sets of columns (samples) considered to be distinct groups for analysis.
If only
one
analysis group is to be used as the control samples (one set of control sample reference computation method), then the values for each column in this parameter field that indicate controls should be specified in the
Control Levels
field.
If
multiple
sets of controls are to be used for different groups of samples, then a subset of the columns specified here need to be specified to define the analysis groups that are control sets in the
Variables Defining Control Sets
field.
For
paired sample subtraction
, this field should contain two variables, one identifying the paired samples and the second that identifies control measurements within the paired samples. The control identifying variable should the only variable specified in the
Variables Defining Control Sets
field.
Note
: For more complex designs, such as nested designs or where the value of the control changes across Analysis groups, it might be necessary to construct a new column. This column is to be included in this field and in the
Variables Defining Control Sets
field, in the EDDS, to indicate the analysis groups that should be used as respective controls for the groups’ experimental samples.