Tip: You can perform a fast rank-based test when the trait is binary, and the variable threshold approach is not used, by checking the Use rank-based statistic for tests box and entering a 0 for this parameter. Also, when the C-alpha test is selected, an asymptotic version of it can be performed by setting this parameter to 0, otherwise a permutation-based test is performed using the number of permutations specified to assess the significance of the C-alpha statistic.