Manhattan Plot (Multiple SNP-Trait Analysis)

A Manhattan Plot tab with genes grouped by chromosome is shown below:

The Manhattan Plot tab contains the following elements:

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One or more Manhattan plots.

When there are multiple annotation plotting groups such as chromosomes, a Manhattan plot is used to show p-values for all genes (or other type of annotation analysis groups) from a particular type of test in a single scatter plot.

The Y-axis variable is the p-value from the association test, converted to the -log or -log10 scale if selected in the process dialog. The X-axis plots the genes in cumulative order, according to the location of the first SNP in the gene, within and across annotation plotting groups.

Each annotation plotting group is assigned a color that is displayed in a legend below the plots.

A horizontal reference line is drawn as a red, dashed line at the significance level that was specified. For -log or -log10-converted p-values, genes above this line are significant; for p-values on the original scale, genes below the line are significant.

A separate plot is created for each association test performed (plots for two different tests are shown above), and if any BY variables were selected, a separate chart is displayed for each BY group as well.

On this plot or any of the other p-value plots, you can mouse-over any of the points on the plot to see the gene's label. When an annotation accession variable is specified when running the process, you can select a point and click on any of the Annotation action buttons - GenBank Nucleotide, UniGene Database, AceView Database, or dbSNP - to link directly to the corresponding website to view extensive annotation information about the gene's first locus.

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The Local Data Filter to the right of the plot can be used to display a single annotation group at a time. Note however that this does not actually select markers for the drill-down buttons. Markers can be selected either by clicking on annotation groups in the legend below the plot or by selecting points in the plot using left-click with the mouse. If any BY variables were selected, a separate set of plots is displayed for each BY group.