Process Description

ANOVA Normalization

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The ANOVA Normalization process normalizes data by fitting an analysis-of-variance linear model across all of the observations in an experiment, and then subtracting the fitted model from the data. The resulting residuals are normalized for the effects that you include in the model. This type of normalization is a natural precursor to individual-level ANOVAs for example, analyzing microarray data gene-by-gene. This process creates an output SAS data set of the same structure as the input data but with normalized response variables. It also produces .html tables describing the results of the SAS PROC MIXED analysis.

What do I need?

Two data sets are required to run this process.

The first data set, the Input Data Set, contains all of the numeric data to be analyzed. This data set must be in the tall format where each sample corresponds to one row and each column corresponds to a separate experimental condition or array.

The drosophilaaging.sas7bdat data set, shown below, is a normalized data set derived from the Drosophila Aging experiment described in Sample Case Studies. It has 49 columns and 100 rows corresponding to 49 arrays and 100 individual probes, respectively.

The second data set is the Experimental Design Data Set (EDDS). This required data set tells how the experiment was performed, providing information about the columns in the input data set. Note that one column in the EDDS must be named ColumnName and the values contained in this column must exactly match the column names in the input data set.

The drosophilaaging_exp.sas7bdat EDDS, is shown below. Note that the ColumnName column lists the column names in the input data set. The Array column corresponds to an index variable. Note the variables describing experimental conditions.

The drosophilaaging.sas7bdat and drosophilaaging_exp.sas7bdat data sets are included in the Sample Data folder.

For detailed information about the files and data sets used or created by JMP Genomics software, see Files and Data Sets.

Output/Results

Refer to the ANOVA Normalization output documentation for detailed descriptions of the output of this process.